Mouse Anti-GM3 Recombinant Antibody (clone 14F7) (CAT#: PABC-051)

Recombinant Mouse Antibody (14F7) is capable of binding to GM3, expressed in Chinese Hamster Ovary cells (CHO). The murine monoclonal antibody 14F7 specifically recognizes N-glycolyl
GM3 and shows no cross-reactivity with the abundant N-acetyl GM3 ganglioside, a close structural homologue of N-glycolyl GM3. And 14F7 strongly recognizes human melanoma and breast cancer tissues.

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SDS-PAGE

Figure 1 Mouse Anti-GM3 Recombinant Antibody (PABC-051) in SDS-PAGE

Figure 1 Mouse Anti-GM3 Recombinant Antibody (PABC-051) in SDS-PAGE

SDS-PAGE analysis of PABC-051 in non-reduced (Lane 1) and reduced (Lane 2) conditions.

SEC-HPLC

Figure 2 Mouse Anti-GM3 Recombinant Antibody (PABC-051) in HPLC

Figure 2 Mouse Anti-GM3 Recombinant Antibody (PABC-051) in HPLC

HPLC analysis of PABC-051 shows the purity is>95%.


Specifications

  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Clone
  • 14F7
  • Applications
  • ELISA

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody 14F7 has been reported in applications of Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry. It's recommended that the optimal antibody concentration, dilution, incubition time etc. be carefully titrated in specific assays.

Target

  • Alternative Names
  • Ganglioside GM3; GM3; monosialodihexosylganglioside

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# PABC-051, RRID: AB_3111575)

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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